Anticorps Monoclonal anti-RAD51

• Phare
• Validé par KD/KO

RAD51 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

4A12B8

N° de cat : 67024-1-PBS

Synonymes

4A12B8, BRCC5, HRAD51, HsRad51, HsT16930

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Galerie de données de validation

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  WB analysis using 67024-1-Ig (same clone as 67024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67024-1-Ig (RAD51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67024-1-Ig (same clone as 67024-1-PBS)

  WB result of RAD51 antibody (67024-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67024-1-Ig (same clone as 67024-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67024-1-Ig (RAD51 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67024-1-Ig (same clone as 67024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67024-1-Ig (RAD51 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67024-1-Ig (same clone as 67024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67024-1-Ig (RAD51 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67024-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67024-1-PBS cible RAD51 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAD51 Protéine recombinante Ag7410
• Nom complet: RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC001459
• Symbole du gène: RAD51
• Identification du gène (NCBI): 5888
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAD51 plays a critical role in the maintenance of genomic integrity by functioning in the repair of DNA double-strand breaks. It mediates homologous pairing and strand exchange in recombinatory structures known as RAD51foci in the nucleus. It also can bind to single and double stranded DNA and exhibits DNA-dependent ATPase activity. In addition, it has a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

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