Anticorps Polyclonal de lapin anti-RAD51AP1

• Phare
• Validé par KD/KO

RAD51AP1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11255-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 11255-1-AP (same clone as 11255-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 11255-1-AP (same clone as 11255-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 11255-1-AP (same clone as 11255-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11255-1-AP (same clone as 11255-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11255-1-AP (same clone as 11255-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11255-1-AP (RAD51AP1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11255-1-AP (same clone as 11255-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11255-1-AP (RAD51AP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11255-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11255-1-PBS cible RAD51AP1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD51AP1 Protéine recombinante Ag1781
• Nom complet: RAD51 associated protein 1
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC016330
• Symbole du gène: RAD51AP1
• Identification du gène (NCBI): 10635
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].

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