Anticorps Recombinant de lapin anti-RAD51C

• Phare
• Validé par KD/KO

RAD51C Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241971D4

Avis (1)

N° de cat : 84537-1-RR

Synonymes

241971D4

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Galerie de données de validation

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  WB analysis of MCF-7 using 84537-1-RR

  WB result of RAD51C antibody (84537-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51C transfected MCF-7 cells.

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  WB analysis using 84537-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84537-1-RR (RAD51C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 84537-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RAD51C Recombinant antibody (84537-1-RR, Clone:241971D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84537-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84537-1-RR against Human RAD51C were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules MCF-7, cellules MDA-MB-231, cellules T-47D
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84537-1-RR cible RAD51C dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAD51C Protéine recombinante Ag35070
• Nom complet: RAD51 homolog C (S. cerevisiae)
• Masse moléculaire calculée: 376 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC107753
• Symbole du gène: RAD51C
• Identification du gène (NCBI): 5889
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jehan (Verified Customer) (05-27-2025)

Antibody produced a clear band at the right size for the desired protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 in 5% milk in TBST
• Cell Tissue Type: OVCAR8 cell

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