Anticorps Polyclonal de lapin anti-RAD52

• Phare
• Validé par KD/KO

RAD52 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 28045-1-AP

Synonymes

DNA repair protein RAD52 homolog

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Galerie de données de validation

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  WB analysis using 28045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28045-1-AP (RAD52 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28045-1-AP

  WB result of RAD52 antibody (28045-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with negative control and RAD52 knockout HeLa cells.

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  WB analysis of HEK-293 using 28045-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 28045-1-AP (RAD52 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 28045-1-AP

  IP result of anti-RAD52 (IP:28045-1-AP, 4ug; Detection:28045-1-AP 1:1000) with HEK-293 cells lysate 2960 ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules HL-60
• Résultats positifs en IP: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below

Informations sur le produit

28045-1-AP cible RAD52 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD52 Protéine recombinante Ag27619
• Nom complet: RAD52 homolog (S. cerevisiae)
• Masse moléculaire calculée: 46 aa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: NM_001297419
• Symbole du gène: RAD52
• Identification du gène (NCBI): 5893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	Mol Cell; EXO1 protects BRCA1-deficient cells against toxic DNA lesions; Authors - Bert van de Kooij
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	WB	J Cell Mol Med; RDM1 promotes critical processes in breast cancer tumorigenesis.; Authors - Yajun Chen
pig	WB	Int J Biochem Cell Biol; Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.; Authors - Guoling Li
human	WB	Cell Death Dis; SF3B4 promotes ovarian cancer progression by regulating alternative splicing of RAD52.; Authors - Yuchao Diao ; KD Validated
human	WB	Nat Commun; Pathway choice in the alternative telomere lengthening in neoplasia is dictated by replication fork processing mediated by EXD2's nuclease activity; Authors - Ronan Broderick ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (10-15-2024)

I detected the expected band for RAD52 and 48 kDa in WT cells, but not RAD52 KO cells, proving that the antibody is indeed recognizing RAD52.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116 and RPE1 cells

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