Anticorps Monoclonal anti-RAD52

RAD52 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3H8G9

N° de cat : 68283-1-PBS

Synonymes

DNA repair protein RAD52 homolog, 3H8G9

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Galerie de données de validation

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  WB analysis using 68283-1-Ig (same clone as 68283-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68283-1-Ig (RAD52 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68283-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68283-1-PBS cible RAD52 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAD52 Protéine recombinante Ag27665
• Nom complet: RAD52 homolog (S. cerevisiae)
• Masse moléculaire calculée: 46 aa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: NM_001297419
• Symbole du gène: RAD52
• Identification du gène (NCBI): 5893
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

In Saccharomyces cerevisiae, Rad52 functions as a critical mediator of homologous recombination (HR) by facilitating the loading of Rad51 onto ssDNA. However, in mammalian systems and other vertebrates, BRCA2 is known to function as the primary mediator of RAD51 filament formation, while prior studies have shown a limited role for RAD52 in HR.Additionally, loss of RAD52 has little consequence for cell viability. Recent evidence suggests that RAD52 may provide an alternative mediator pathway to BRCA2 function (PMID: 34928169). Several studies have suggested a link between the functional regulation of RAD52 and the protein modification by a small ubiquitin-like modifier (SUMO), sumoylation may play an important role in the nuclear transport of RAD52 (PMID:20190268). The calculated MW of Rad52 is 46 kDa, 68283-1-Ig can detect the band around 46 kDa.

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