Anticorps Polyclonal de lapin anti-RAD9A

RAD9A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13035-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 13035-1-AP (same clone as 13035-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 13035-1-AP (same clone as 13035-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 13035-1-AP (same clone as 13035-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13035-1-AP (same clone as 13035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13035-1-AP (same clone as 13035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13035-1-AP (same clone as 13035-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13035-1-AP (RAD9A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13035-1-PBS cible RAD9A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD9A Protéine recombinante Ag3687
• Nom complet: RAD9 homolog A (S. pombe)
• Masse moléculaire calculée: 391 aa, 42 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC014848
• Symbole du gène: RAD9A
• Identification du gène (NCBI): 5883
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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