Anticorps Polyclonal de lapin anti-RADIL

• Phare
• Validé par KD/KO

RADIL Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20284-1-PBS

Synonymes

KIAA1849, Ras-associating and dilute domain-containing protein

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Galerie de données de validation

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  WB analysis using 20284-1-AP (same clone as 20284-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20284-1-AP (RADIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20284-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 20284-1-AP (same clone as 20284-1-PBS)

  IP result of anti-RADIL (IP:20284-1-AP, 4ug; Detection:20284-1-AP 1:1000) with HEK-293 cells lysate 1400 ug. This data was developed using the same antibody clone with 20284-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20284-1-PBS cible RADIL dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RADIL Protéine recombinante Ag13996
• Nom complet: Ras association and DIL domains
• Masse moléculaire calculée: 1075 aa, 117 kDa
• Poids moléculaire observé: 117 kDa
• Numéro d’acquisition GenBank: BC004919
• Symbole du gène: RADIL
• Identification du gène (NCBI): 55698
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Radil is a broadly expressed adapter protein that contains a RA-like domain, a PDZ domain and a DIL domain. Like RAPL and RIAM, Radil interacts preferentially with active GTP-bound Rap1, and overexpression of Radil enhances integrin-mediated adhesion. In addition, Radil knockdown inhibits cell-substrate adhesion in HT1080, AD293, and NMuMG cells and results in migration defects in SW1353 and zebrafish neural crest cells (PMID: 17704304). Radil regulates neutrophil adhesion by specifically enhancing β2-integrin activation, as well as focal adhesion kinase (FAK) and paxillin phosphorylation (PMID: 23097489).

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