Anticorps Recombinant de lapin anti-RAE1

RAE1 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240861E12

N° de cat : 83712-1-PBS

Synonymes

240861E12, MIG14, Mnrp41, mRNA export factor, mRNA export factor RAE1

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Galerie de données de validation

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  WB analysis using 83712-1-RR (same clone as 83712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83712-1-RR (RAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83712-1-RR (same clone as 83712-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAE1 Recombinant antibody (83712-1-RR, Clone:240861E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83712-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83712-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83712-1-PBS against Human RAE1 was performed. The affinity constant is 1.14 nM.

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  Affinity and Kinetic Characterization of 83712-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83712-1-RR against Human RAE1 were performed. The affinity constant is 1.36 nM.

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Informations sur le produit

83712-1-PBS cible RAE1 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAE1 Protéine recombinante Ag12102
• Nom complet: RAE1 RNA export 1 homolog (S. pombe)
• Masse moléculaire calculée: 368 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC103754
• Symbole du gène: RAE1
• Identification du gène (NCBI): 8480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)

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