Anticorps Recombinant de lapin anti-RAE1

RAE1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240861B7

N° de cat : 83712-4-PBS

Synonymes

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

---

Galerie de données de validation

• 

  WB analysis using 83712-4-RR (same clone as 83712-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83712-4-RR (RAE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 83712-4-RR (same clone as 83712-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83712-4-RR (RAE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83712-4-RR (same clone as 83712-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAE1 antibody (83712-4-RR, Clone: 240861B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83712-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83712-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83712-4-RR against Human RAE1 were performed. The affinity constant is 20.7 nM.

---

Informations sur le produit

83712-4-PBS cible RAE1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAE1 Protéine recombinante Ag12102
• Nom complet: RAE1 RNA export 1 homolog (S. pombe)
• Masse moléculaire calculée: 368 aa, 41 kDa
• Numéro d’acquisition GenBank: BC103754
• Symbole du gène: RAE1
• Identification du gène (NCBI): 8480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)

{{ptg:RelatedPrimaryAntibodies}}