Anticorps Polyclonal de lapin anti-RAF1

RAF1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 26863-1-PBS

Synonymes

Proto-oncogene c-RAF, Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF

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Galerie de données de validation

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  WB analysis of DU 145 using 26863-1-AP (same clone as 26863-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 26863-1-AP (RAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26863-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 26863-1-AP (same clone as 26863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26863-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 26863-1-AP (same clone as 26863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26863-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 26863-1-AP (same clone as 26863-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26863-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26863-1-PBS cible RAF1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAF1 Protéine recombinante Ag25423
• Nom complet: v-raf-1 murine leukemia viral oncogene homolog 1
• Masse moléculaire calculée: 648 aa, 73 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC018119
• Symbole du gène: RAF1
• Identification du gène (NCBI): 5894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

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