Anticorps Monoclonal anti-RAF1

• Phare
• Validé par KD/KO

RAF1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D6A1

N° de cat : 66592-1-PBS

Synonymes

Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf

---

Galerie de données de validation

• 

  WB analysis using 66592-1-Ig (same clone as 66592-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66592-1-Ig (same clone as 66592-1-PBS)

  WB result of RAF1 antibody (66592-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DC2.4 using 66592-1-Ig (same clone as 66592-1-PBS)

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66592-1-Ig (RAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66592-1-Ig (same clone as 66592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66592-1-Ig (same clone as 66592-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66592-1-Ig (RAF1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 66592-1-Ig (same clone as 66592-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RAF1 antibody (66592-1-Ig, Clone: 1D6A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66592-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66592-1-PBS cible RAF1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAF1 Protéine recombinante Ag25423
• Nom complet: v-raf-1 murine leukemia viral oncogene homolog 1
• Masse moléculaire calculée: 648 aa, 73 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC018119
• Symbole du gène: RAF1
• Identification du gène (NCBI): 5894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

{{ptg:RelatedPrimaryAntibodies}}