Anticorps Recombinant de lapin anti-RAF1

RAF1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242540A2

N° de cat : 84994-4-PBS

Synonymes

Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf

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Galerie de données de validation

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  WB analysis using 84994-4-RR (same clone as 84994-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84994-4-RR (RAF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84994-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84994-4-RR (same clone as 84994-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAF1 antibody (84994-4-RR, Clone: 242540A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84994-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84994-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84994-4-RR against Human RAF1 were performed. The affinity constant is 2.84 nM.

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Informations sur le produit

84994-4-PBS cible RAF1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAF1 Protéine recombinante Ag25423
• Nom complet: v-raf-1 murine leukemia viral oncogene homolog 1
• Masse moléculaire calculée: 648 aa, 73 kDa
• Poids moléculaire observé: ~75 kDa
• Numéro d’acquisition GenBank: BC018119
• Symbole du gène: RAF1
• Identification du gène (NCBI): 5894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

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