Anticorps Polyclonal de lapin anti-RAG2

RAG2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 11825-1-AP

Synonymes

RAG 2, RAG2

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Galerie de données de validation

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  WB analysis of A375 using 11825-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11825-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11825-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11825-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11825-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A375 using 11825-1-AP

  IP Result of anti-RAG2 (IP:11825-1-AP, 4ug; Detection:11825-1-AP 1:300) with A375 cells lysate 3600ug.

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  IHC staining of human lymphoma using 11825-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11825-1-AP (RAG2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11825-1-AP

  Immunofluorescent analysis of Hela cells, using RAG2 antibody 11825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 11825-1-AP

  1X10^6 HeLa cells were stained with 0.2ug RAG2 antibody (11825-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A375, tissu de thymus de souris
• Résultats positifs en IP: cellules A375
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

11825-1-AP cible RAG2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAG2 Protéine recombinante Ag2393
• Nom complet: recombination activating gene 2
• Masse moléculaire calculée: 527 aa, 59 kDa
• Poids moléculaire observé: 57-62 kDa
• Numéro d’acquisition GenBank: BC022397
• Symbole du gène: RAG2
• Identification du gène (NCBI): 5897
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3

Publications

Species	Application	Title
mouse	WB	Nat Commun; The 3D enhancer network of the developing T cell genome is shaped by SATB1; Authors - Tomas Zelenka
mouse	WB	J Immunol; Allelic Exclusion of IgH through Inhibition of E2A in a VDJ Recombination Complex.; Authors - Jannek Hauser