Anticorps Monoclonal anti-RAG2

RAG2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1B4E5

N° de cat : 66998-1-Ig

Synonymes

1B4E5, RAG 2, RAG-2, V(D)J recombination-activating protein 2

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Galerie de données de validation

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  WB analysis using 66998-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66998-1-Ig (RAG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66998-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAG2 antibody (66998-1-Ig, Clone: 1B4E5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66998-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RAG2 (66998-1-Ig, Clone:1B4E5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Raji, cellules Ramos
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66998-1-Ig cible RAG2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAG2 Protéine recombinante Ag16780
• Nom complet: recombination activating gene 2
• Masse moléculaire calculée: 527 aa, 59 kDa
• Poids moléculaire observé: 57-62 kDa
• Numéro d’acquisition GenBank: BC022397
• Symbole du gène: RAG2
• Identification du gène (NCBI): 5897
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3

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