Anticorps Recombinant de lapin anti-RAI14

RAI14 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251495G12

N° de cat : 86581-1-PBS

Synonymes

Ankycorbin, Ankyrin repeat and coiled-coil structure-containing protein, KIAA1334, NORPEG, RAI13

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Galerie de données de validation

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  WB analysis using 86581-1-RR (same clone as 86581-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86581-1-RR (RAI14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86581-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 86581-1-RR (same clone as 86581-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 86581-1-RR (RAI14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86581-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86581-1-RR (same clone as 86581-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAI14 antibody (86581-1-RR, Clone: 251495G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86581-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86581-1-PBS cible RAI14 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAI14 Protéine recombinante Ag11708
• Nom complet: retinoic acid induced 14
• Masse moléculaire calculée: 980 aa, 110 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC052988
• Symbole du gène: RAI14
• Identification du gène (NCBI): 26064
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAI14 (retinoic acid-induced protein 14, also called ankycorbin or NORPEG) was originally identified in human retinal pigment epithelial cells as a retinoic-acid-responsive gene. Through direct interaction with actin-binding partners such as palladin and TARA, RAI14 orchestrates focal adhesion dynamics, cell polarity and mechano-sensing; in epithelia it stabilizes tight-junction complexes, whereas in neurons it localizes to the dendritic-spine neck where it promotes spine maintenance by antagonizing F-actin depolymerization. Consistent with its cytoskeletal role, RAI14 has emerged as a context-dependent modulator of several signalling cascades.

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