Anticorps Polyclonal de lapin anti-RALA

• Phare
• Validé par KD/KO

RALA Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 13629-1-AP

Synonymes

RAL, RALA, Ras related protein Ral A

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Galerie de données de validation

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  WB analysis of mouse brain using 13629-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13629-1-AP

  WB result of RALA antibody (13629-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RALA transfected HeLa cells.

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  WB analysis of rat brain using 13629-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13629-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 13629-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of MCF-7 using 13629-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13629-1-AP (RALA Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human breast cancer using 13629-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13629-1-AP (RALA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13629-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13629-1-AP (RALA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules MCF-7, tissu cérébral de rat, tissu cérébral humain, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below

Informations sur le produit

13629-1-AP cible RALA dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RALA Protéine recombinante Ag4547
• Nom complet: v-ral simian leukemia viral oncogene homolog A (ras related)
• Masse moléculaire calculée: 206 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC039858
• Symbole du gène: RALA
• Identification du gène (NCBI): 5898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RALA belongs to the small GTPase superfamily. Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. RALA plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. As an effector of the proto-oncogene Ras, it play an important role in the molecular pathology of human malignancies.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
human	WB	J Extracell Vesicles; A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.; Authors - Charles P Hinzman
human	WB, IHC	Front Chem; Design, Synthesis, and Biological Evaluation of Pyrano[2,3-c]-pyrazole-Based RalA Inhibitors Against Hepatocellular Carcinoma.; Authors - Yuting Wang
human	IHC	Hum Pathol; Papillary renal neoplasm with reverse polarity: a clinicopathologic study of 43 cases with a focus on the expression of KRAS signaling pathway downstream effectors; Authors - Bohyun Kim
human	WB	Intractable Rare Dis Res; A comparative proteomics study on matrix vesicles of osteoblast-like Saos-2 and U2-OS cells.; Authors - Liang Jiang
mouse	WB	Oncotarget; Evaluation of cancer-derived myocardial impairments using a mouse model.; Authors - Yoshihiro Miyagawa