Anticorps Monoclonal anti-RALBP1

RALBP1 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B2F5

N° de cat : 67359-1-PBS

Synonymes

DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein, 76 kDa Ral interacting protein

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 67359-1-Ig (same clone as 67359-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67359-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67359-1-Ig (same clone as 67359-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67359-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67359-1-Ig (same clone as 67359-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67359-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67359-1-Ig (same clone as 67359-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67359-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67359-1-Ig (same clone as 67359-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67359-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 67359-1-Ig (same clone as 67359-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug RALBP1 Monoclonal antibody (67359-1-Ig, Clone:1B2F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67359-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67359-1-PBS cible RALBP1 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RALBP1 Protéine recombinante Ag28618
• Nom complet: ralA binding protein 1
• Masse moléculaire calculée: 655 aa, 76 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC013126
• Symbole du gène: RALBP1
• Identification du gène (NCBI): 10928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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