Anticorps Monoclonal anti-RALY

RALY Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10C3

N° de cat : CL488-68011

Synonymes

Autoantigen p542, hnRNP core protein C like 2, HNRPCL2, P542, RALY, RNA binding protein Raly

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Galerie de données de validation

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  IF Staining of A431 using CL488-68011

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RALY antibody (CL488-68011, Clone: 2B10C3 ) at dilution of 1:600.

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  FC experiment of A431 using CL488-68011

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALY (CL488-68011, Clone:2B10C3) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of A431 using CL488-68011

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALY (CL488-68011, Clone:2B10C3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules A431,
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:300-1:1200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-68011 cible RALY dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RALY Protéine recombinante Ag12250
• Nom complet: RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
• Masse moléculaire calculée: 307 aa, 33 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC103753
• Symbole du gène: RALY
• Identification du gène (NCBI): 22913
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.