Anticorps Monoclonal anti-RALYL

RALYL Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1F3F4

N° de cat : 67534-1-Ig

Synonymes

1F3F4, Heterogeneous nuclear ribonucleoprotein C-like 3, hnRNP core protein C like 3, hnRNP core protein C-like 3, HNRPCL3

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Galerie de données de validation

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  WB analysis using 67534-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67534-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67534-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67534-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67534-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67534-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67534-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67534-1-Ig (RALYL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 67534-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67534-1-Ig (RALYL antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules Daudi, cellules HEK-293, cellules HeLa, cellules HepG2, cellules LNCaP, cellules Raji, tissu cérébral de lapin, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

67534-1-Ig cible RALYL dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RALYL Protéine recombinante Ag11676
• Nom complet: RALY RNA binding protein-like
• Masse moléculaire calculée: 291 aa, 32 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC031090
• Symbole du gène: RALYL
• Identification du gène (NCBI): 138046
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Cancer Rep (Hoboken); RALYL Overexpression Suppresses Colorectal Cancer via Modulating HNRNPC-Mediated MNK2 Alternative Splicing; Authors - Zenghui Ma

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