Anticorps Polyclonal de lapin anti-RAN

• Phare
• Validé par KD/KO

RAN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(19)

Avis (1)

N° de cat : 10469-1-AP

Synonymes

ARA24, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran, GTPase Ran

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Galerie de données de validation

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  WB analysis using 10469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10469-1-AP

  WB result of RAN antibody (10469-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells.

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  WB analysis of HeLa using 10469-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10469-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 10469-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10469-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10469-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 10469-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10469-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10469-1-AP

  IP result of anti-RAN (IP:10469-1-AP, 4ug; Detection:10469-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human breast cancer using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded normal colon slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10469-1-AP (RAN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10469-1-AP (RAN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10469-1-AP (RAN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10469-1-AP (RAN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 10469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RAN antibody (10469-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 10469-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RAN Polyclonal antibody (10469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HeLa using 10469-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAN Polyclonal antibody (10469-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules NIH/3T3, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de côlon de rat, tissu de côlon de souris, tissu de côlon humain, tissu placentaire humain, tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,
• Résultats positifs en FC (Intra): cellules HeLa, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 3 publications below

Informations sur le produit

10469-1-AP cible RAN dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris, Macrobrachium Rosenbergii
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAN Protéine recombinante Ag0748
• Nom complet: RAN, member RAS oncogene family
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC004272
• Symbole du gène: RAN
• Identification du gène (NCBI): 5901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IP,IF	Cell Death Dis; RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.; Authors - Xunhua Liu ; KD Validated
rat,human	WB	Diabetologia; Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.; Authors - Mu Zhang
rat	WB,IP	Cell Commun Signal; Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.; Authors - Abiola Abdulrahman Ayanlaja
human	IHC	Oncotarget; Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS.; Authors - Zhen Chen
human	WB	Int J Biol Sci; Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.; Authors - Yancheng Zhong ; KD Validated
human	IF	J Cell Sci; A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex.; Authors - Mohamed Hamed

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works very well for WB (1:1000, 1h) and IF in permeabilized cells (1:200). Validated with siRNA

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC

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