Anticorps Polyclonal de lapin anti-RAN

• Phare
• Validé par KD/KO

RAN Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (1)

N° de cat : 10469-1-AP

Synonymes

ARA24, Gsp1, GTPase Ran, RAN, Ras like protein TC4, Ras related nuclear protein, TC4

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Galerie de données de validation

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  WB analysis of HEK-293 using 10469-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 10469-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10469-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10469-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10469-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10469-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10469-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10469-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10469-1-AP (RAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10469-1-AP

  IP Result of anti-RAN (IP:10469-1-AP, 4ug; Detection:10469-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10469-1-AP (RAN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10469-1-AP (RAN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10469-1-AP (RAN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10469-1-AP (RAN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10469-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10469-1-AP (RAN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10469-1-AP (RAN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAN antibody (10469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A375, cellules HeLa, cellules Jurkat, cellules NIH/3T3, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

10469-1-AP cible RAN dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Macrobrachium Rosenbergii
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAN Protéine recombinante Ag0748
• Nom complet: RAN, member RAS oncogene family
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC004272
• Symbole du gène: RAN
• Identification du gène (NCBI): 5901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF,IP	Cell Death Dis; RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.; Authors - Xunhua Liu ; KD Validated
human,rat	WB	Diabetologia; Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.; Authors - Mu Zhang
rat	IP,WB	Cell Commun Signal; Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression.; Authors - Abiola Abdulrahman Ayanlaja
human	IHC	Oncotarget; Identification of nasopharyngeal carcinoma metastasis-related biomarkers by iTRAQ combined with 2D-LC-MS/MS.; Authors - Zhen Chen
human	WB	Int J Biol Sci; Lin28A Regulates Stem-like Properties of Ovarian Cancer Cells by Enriching RAN and HSBP1 mRNA and Up-regulating its Protein Expression.; Authors - Yancheng Zhong ; KD Validated
human	IF	J Cell Sci; A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex.; Authors - Mohamed Hamed

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works very well for WB (1:1000, 1h) and IF in permeabilized cells (1:200). Validated with siRNA

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC