Anticorps Monoclonal anti-RAN

• Phare
• Validé par KD/KO

RAN Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B11E1

N° de cat : 67500-1-PBS

Synonymes

ARA24, 1B11E1, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran

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Galerie de données de validation

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  WB analysis using 67500-1-Ig (same clone as 67500-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67500-1-Ig (RAN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67500-1-Ig (same clone as 67500-1-PBS)

  WB result of RAN antibody (67500-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells. This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RAN antibody (67500-1-Ig, Clone: 1B11E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67500-1-PBS cible RAN dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAN Protéine recombinante Ag30034
• Nom complet: RAN, member RAS oncogene family
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC004272
• Symbole du gène: RAN
• Identification du gène (NCBI): 5901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).

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