Anticorps Recombinant de lapin anti-RAN

• Phare
• Validé par KD/KO

RAN Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230317F3

N° de cat : 82978-1-PBS

Synonymes

ARA24, 230317F3, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran

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Galerie de données de validation

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  WB analysis using 82978-1-RR (same clone as 82978-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82978-1-RR (RAN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 82978-1-RR (same clone as 82978-1-PBS)

  WB result of RAN antibody (82978-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 82978-1-RR (same clone as 82978-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 82978-1-RR (same clone as 82978-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82978-1-RR (same clone as 82978-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82978-1-RR (same clone as 82978-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82978-1-RR (same clone as 82978-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAN antibody (82978-1-RR, Clone: 230317F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 82978-1-RR (same clone as 82978-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82978-1-RR (same clone as 82978-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82978-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82978-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82978-1-RR against Human RAN were performed. The affinity constant is 3.08 nM.

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Informations sur le produit

82978-1-PBS cible RAN dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAN Protéine recombinante Ag0748
• Nom complet: RAN, member RAS oncogene family
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC004272
• Symbole du gène: RAN
• Identification du gène (NCBI): 5901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).

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