Anticorps Recombinant de lapin anti-RAN

• Phare
• Validé par KD/KO

RAN Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230317F3

N° de cat : 82978-1-RR

Synonymes

ARA24, 230317F3, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran

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Galerie de données de validation

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  WB analysis using 82978-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82978-1-RR (RAN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82978-1-RR

  WB result of RAN antibody (82978-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells.

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  IHC staining of rat kidney using 82978-1-RR

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 82978-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 82978-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 82978-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82978-1-RR (RAN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82978-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAN antibody (82978-1-RR, Clone: 230317F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A431 using 82978-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of HeLa using 82978-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human RAN (82978-1-RR, Clone:230317F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 82978-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82978-1-RR against Human RAN were performed. The affinity constant is 3.08 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules C6, cellules HEK-293, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IHC: tissu de côlon humain, tissu placentaire humain, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82978-1-RR cible RAN dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAN Protéine recombinante Ag0748
• Nom complet: RAN, member RAS oncogene family
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC004272
• Symbole du gène: RAN
• Identification du gène (NCBI): 5901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).

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