Anticorps Polyclonal de lapin anti-RANBP9

• Phare
• Validé par KD/KO

RANBP9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 17755-1-AP

Synonymes

BPM L, BPM90, RAN binding protein 9, Ran binding protein M, RanBP7, RANBP9, RANBPM

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Galerie de données de validation

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  WB analysis of HeLa using 17755-1-AP

  WB result of RANBP9 antibody (17755-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANBP9 transfected HeLa cells.

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  WB analysis using 17755-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17755-1-AP (RANBP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 17755-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17755-1-AP (RANBP9 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 17755-1-AP

  IP Result of anti-RANBP9 (IP:17755-1-AP, 4ug; Detection:17755-1-AP 1:600) with HeLa cells lysate 1600ug.

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  IHC staining of human testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of rat testis using 17755-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17755-1-AP (RANBP9 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MDCK using 17755-1-AP

  Immunofluorescent analysis of MDCK cells using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 17755-1-AP

  Immunofluorescent analysis of HeLa cells using 17755-1-AP (RANBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, testicule de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain, tissu cutané humain, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MDCK, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

17755-1-AP cible RANBP9 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RANBP9 Protéine recombinante Ag12112
• Nom complet: RAN binding protein 9
• Masse moléculaire calculée: 388aa,43 kDa; 729aa,78 kDa
• Poids moléculaire observé: 80-90 kDa
• Numéro d’acquisition GenBank: BC063849
• Symbole du gène: RANBP9
• Identification du gène (NCBI): 10048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	mSphere; B Cell Receptor-Responsive miR-141 Enhances Epstein-Barr Virus Lytic Cycle via FOXO3 Inhibition.; Authors - Yan Chen
human	WB	Oncol Lett; RANBP9 suppresses tumor proliferation in colorectal cancer.; Authors - Chunzhi Qin
rat	IF	Acta Histochem; MicroRNA-26a-5p alleviates neuronal apoptosis and brain injury in intracerebral hemorrhage by targeting RAN binding protein 9.; Authors - Huiyuan Zhang
human	IHC	Transl Cancer Res; Reduced RANBP9 expression is associated with poor prognosis in colorectal cancer patients.; Authors - Guangbin Wu