Anticorps Monoclonal anti-RanGAP1

RanGAP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F3A4

N° de cat : 67146-1-PBS

Synonymes

1F3A4, KIAA1835, Ran GTPase-activating protein 1, SD

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Galerie de données de validation

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  WB analysis of HeLa using 67146-1-Ig (same clone as 67146-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

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  WB analysis using 67146-1-Ig (same clone as 67146-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67146-1-Ig (same clone as 67146-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 67146-1-Ig (same clone as 67146-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67146-1-Ig (same clone as 67146-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67146-1-Ig (same clone as 67146-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67146-1-Ig (same clone as 67146-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67146-1-Ig (same clone as 67146-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67146-1-Ig (RANGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67146-1-Ig (same clone as 67146-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67146-1-Ig (RANGAP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67146-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67146-1-PBS cible RanGAP1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RanGAP1 Protéine recombinante Ag26136
• Nom complet: Ran GTPase activating protein 1
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa, 82 kDa
• Numéro d’acquisition GenBank: BC014044
• Symbole du gène: RANGAP1
• Identification du gène (NCBI): 5905
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RanGAP1 is a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. In mammalian cells, unmodified RanGAP1 is predominantly cytoplasmic, whereas modification by small ubiquitin-related modifier protein (SUMO) targets RanGAP1 to the cytoplasmic filaments of nuclear pore complex (NPC) where it forms a complex with RanBP2 and Ubc9. Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity. We got 64 kDa (cytoplasmic Ran GAP1) and 82 kDa (SUMO-1 modified Ran GAP1) in western blotting.

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