Anticorps Polyclonal de lapin anti-MOG1

MOG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 10552-1-AP

Synonymes

Ran-binding protein MOG1, RanGNRF

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Galerie de données de validation

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  WB analysis of SGC-7901 using 10552-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10552-1-AP (MOG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10552-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10552-1-AP (MOG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10552-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10552-1-AP (MOG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10552-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10552-1-AP (MOG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (MOG1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10552-1-AP (MOG1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10552-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MOG1 antibody (10552-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

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Applications testées

• Résultats positifs en WB: cellules SGC-7901, cellules HepG2, tissu pulmonaire de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

10552-1-AP cible MOG1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MOG1 Protéine recombinante Ag0824
• Nom complet: RAN guanine nucleotide release factor
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18-22 kDa
• Numéro d’acquisition GenBank: BC006486
• Symbole du gène: RANGRF
• Identification du gène (NCBI): 29098
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RANGRF, also named as MOG1, RANGNRF, HSPC165, HSPC236 and MDS5, regulates the intracellular trafficking of RAN. In cardiac cells it regulates the cell surface localization of SCN5A.

Publications

Species	Application	Title
mouse	IHC	Sci Transl Med; Gene therapy targeting protein trafficking regulator MOG1 in mouse models of Brugada syndrome, arrhythmias, and mild cardiomyopathy.; Authors - Gang Yu
	WB	Function (Oxf); ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet β-Cell; Authors - Kristen E Rohli
		Stem Cell Res; Generation of a homozygous RANGRF knockout hiPSC line by CRISPR/Cas9 system; Authors - Meiling Jiang

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