Anticorps Polyclonal de lapin anti-TNFSF11/RANKL

TNFSF11/RANKL Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(73)

N° de cat : 23408-1-AP

Synonymes

TNFSF11, Tumor necrosis factor ligand superfamily member 11, TNF-related activation-induced cytokine, Receptor activator of nuclear factor kappa-B ligand, CD254

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Galerie de données de validation

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  WB analysis using 23408-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23408-1-AP (RANKL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 23408-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23408-1-AP (RANKL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 23408-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23408-1-AP (RANKL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23408-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23408-1-AP (RANKL antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 23408-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23408-1-AP (RANKL antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 23408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23408-1-AP (RANKL antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules Raji,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu cardiaque humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 48 publications below
• IHC: See 25 publications below
• IF: See 10 publications below

Informations sur le produit

23408-1-AP cible TNFSF11/RANKL dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNFSF11/RANKL Protéine recombinante Ag19975
• Nom complet: tumor necrosis factor (ligand) superfamily, member 11
• Masse moléculaire calculée: 317 aa, 35 kDa
• Poids moléculaire observé: 20-30 kDa
• Numéro d’acquisition GenBank: BC074890
• Symbole du gène: RANKL
• Identification du gène (NCBI): 8600
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Exosomes derived from osteogenic tumor activate osteoclast differentiation and concurrently inhibit osteogenesis by transferring COL1A1-targeting miRNA-92a-1-5p.; Authors - Lijuan Yu
mouse,human	WB	Aging Cell; Pyrroloquinoline quinone alleviates natural aging-related osteoporosis via a novel MCM3-Keap1-Nrf2 axis-mediated stress response and Fbn1 upregulation; Authors - Jie Li
rat	IF	J Control Release; Sialic acid-modified chitosan oligosaccharide-based biphasic calcium phosphate promote synergetic bone formation in rheumatoid arthritis therapy.; Authors - Xiao-Ling Xu
mouse	WB	Antioxid Redox Signal; Titanium Particles Activate Osteocytic Connexin 43 to Induce Oxidative Stress and Osteoclastogenesis Through the JAK-STAT Pathway; Authors - Jiawei Ouyang
mouse	WB	Mol Metab; Loss of chemerin triggers bone remodeling in vivo and in vitro.; Authors - Long Han
mouse	WB	Aging (Albany NY); Increased expression of osteopontin in subchondral bone promotes bone turnover and remodeling, and accelerates the progression of OA in a mouse model.; Authors - Chuangxin Lin

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