Anticorps Monoclonal anti-TNFSF11/RANKL

• Phare
• Validé par KD/KO

TNFSF11/RANKL Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(11)

CloneNo.

3F2E1

N° de cat : 66610-1-Ig

Synonymes

TNFSF11, 3F2E1, CD 254, CD254, Receptor activator of nuclear factor kappa-B ligand

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Galerie de données de validation

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  WB analysis using 66610-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66610-1-Ig

  WB result of RANKL antibody (66610-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANKL transfected HeLa cells.

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  WB analysis of DC2.4 using 66610-1-Ig

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 66610-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66610-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 66610-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 66610-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 66610-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 66610-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RANKL antibody (66610-1-Ig, Clone: 3F2E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules DC2.4, cellules HeLa, cellules HUVEC, cellules JAR, cellules NCCIT, cellules RAW 264.7, cellules U2OS, tissu splénique humain
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

66610-1-Ig cible TNFSF11/RANKL dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TNFSF11/RANKL Protéine recombinante Ag19975
• Nom complet: tumor necrosis factor (ligand) superfamily, member 11
• Masse moléculaire calculée: 317 aa, 35 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC074890
• Symbole du gène: RANKL
• Identification du gène (NCBI): 8600
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.

Publications

Species	Application	Title
mouse	IHC	J Cancer; Gut microbiota deficiency ameliorates multiple myeloma and myeloma-related bone disease by Th17 cells in mice models; Authors - Jia Liu
rat	WB,IF	Folia Histochem Cytobiol; Platelet-rich plasma ameliorates cartilage degradation in rat models of osteoarthritis via the OPG/RANKL/RANK system; Authors - Qun Wu
human	WB,IF	Cell Death Dis; RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis.; Authors - Qian Liang
mouse	WB,IHC,IF	Open Life Sci; Effect of periostin on bone metabolic and autophagy factors during tooth eruption in mice; Authors - Han Qin
mouse	IF	Adv Healthc Mater; Structured Polymers Enable the Sustained Delivery of Glucocorticoids within the Intra-Articular Space; Authors - Ana Crastin
	WB	Poult Sci; Zinc Glycine supplementation improves bone quality in meat geese by modulating gut microbiota, SCFA's, and gut barrier function through Wnt10b/NF-κB axis; Authors - Zeshan Zulfiqar

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