Anticorps Recombinant de lapin anti-RAP1A

• Phare
• Validé par KD/KO

RAP1A Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240438G5

N° de cat : 83708-1-RR

Synonymes

240438G5, EC:3.6.5.2, G-22K, GTP-binding protein smg p21A, KREV1

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Galerie de données de validation

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  WB analysis using 83708-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83708-1-RR (RAP1A antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 83708-1-RR

  WB result of RAP1A antibody (83708-1-RR; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAP1A transfected A431 cells.

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  IF Staining of NIH/3T3 using 83708-1-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAP1A antibody (83708-1-RR, Clone: 240438G5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of HeLa using 83708-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-1-RR, Clone:240438G5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83708-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83708-1-RR against Human RAP1A were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules K-562, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu placentaire humain
• Résultats positifs en IF/ICC: cellules NIH/3T3,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83708-1-RR cible RAP1A dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAP1A Protéine recombinante Ag9488
• Nom complet: RAP1A, member of RAS oncogene family
• Masse moléculaire calculée: 184 aa, 21 kDa
• Numéro d’acquisition GenBank: BC014086
• Symbole du gène: RAP1A
• Identification du gène (NCBI): 5906
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).

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