Anticorps Recombinant de lapin anti-RAP1A

RAP1A Recombinant Antibody for ELISA, FC (Intra), WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

ELISA, FC (Intra), WB

Conjugaison

Non conjugué

CloneNo.

240438E10

N° de cat : 83708-2-PBS

Synonymes

Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2

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Galerie de données de validation

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  WB analysis using 83708-2-RR (same clone as 83708-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83708-2-RR (RAP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83708-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83708-2-RR (same clone as 83708-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-2-RR, Clone:240438E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83708-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83708-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83708-2-RR against Human RAP1A were performed. The affinity constant is 0.232 nM.

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Informations sur le produit

83708-2-PBS cible RAP1A dans les applications de ELISA, FC (Intra), WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAP1A Protéine recombinante Ag9488
• Nom complet: RAP1A, member of RAS oncogene family
• Masse moléculaire calculée: 184 aa, 21 kDa
• Numéro d’acquisition GenBank: BC014086
• Symbole du gène: RAP1A
• Identification du gène (NCBI): 5906
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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