Anticorps Monoclonal anti-RAP1B

RAP1B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E7G11

N° de cat : 67174-1-PBS

Synonymes

2E7G11, EC:3.6.5.2, GTP binding protein smg p21B, GTP-binding protein smg p21B, K REV

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Galerie de données de validation

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  WB analysis using 67174-1-Ig (same clone as 67174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67174-1-Ig (RAP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67174-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67174-1-Ig (same clone as 67174-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67174-1-Ig (RAP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67174-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67174-1-Ig (same clone as 67174-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67174-1-Ig (RAP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67174-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67174-1-Ig (same clone as 67174-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67174-1-Ig (RAP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67174-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 67174-1-Ig (same clone as 67174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67174-1-Ig (RAP1B antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67174-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 67174-1-Ig (same clone as 67174-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67174-1-Ig (RAP1B antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67174-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67174-1-Ig (same clone as 67174-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAP1B antibody (67174-1-Ig, Clone: 2E7G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67174-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67174-1-PBS cible RAP1B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAP1B Protéine recombinante Ag29212
• Nom complet: RAP1B, member of RAS oncogene family
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000176
• Symbole du gène: RAP1B
• Identification du gène (NCBI): 5908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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