Anticorps Recombinant de lapin anti-RAP1GAP

RAP1GAP Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250422E11

N° de cat : 86011-2-PBS

Synonymes

KIAA0474, RAP1 GTPase activating protein, Rap1 GTPase-activating protein 1, RAP1GA1, RAP1GAP1

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Galerie de données de validation

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  WB analysis using 86011-2-RR (same clone as 86011-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86011-2-RR (RAP1GAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86011-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 86011-2-RR (same clone as 86011-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 86011-2-RR (RAP1GAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86011-2-PBS in a different storage buffer formulation.

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Informations sur le produit

86011-2-PBS cible RAP1GAP dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAP1GAP Protéine recombinante Ag5576
• Nom complet: RAP1 GTPase activating protein
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 85-95 kDa
• Numéro d’acquisition GenBank: BC054490
• Symbole du gène: RAP1GAP
• Identification du gène (NCBI): 5909
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rap1 GTPase-activating protein (Rap1GAP) is an important tumor suppressor. Rap1GAP was associated with the AMPK and NF-κB signaling pathways and that Rap1GAP inhibited AMPK/SIRT1 and activated the NF-κB signaling pathway in model animals. Rap1GAP was verified to regulate the AMPK SIRT1/NF-κB signaling pathway and exacerbate the damage to myocardial cells caused by ischemia and hypoxia (PMID: 38320624). Rap1GAP is involved in Ang II-induced cardiomyocyte hypertrophy through the regulation of autophagy (PMID: 33936386). Rap1GAP, which is the GTPase-activating protein of Rap1, can bind to Rap1, promote the transformation of GTP from its active to inactive form, and terminate signal transduction (PMID: 21295042).

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