Anticorps Polyclonal de lapin anti-RAP2A

RAP2A Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 13789-1-AP

Synonymes

K REV, KREV, RAP2, RAP2A, Ras related protein Rap 2a, RbBP 30

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Galerie de données de validation

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  WB analysis of A431 using 13789-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13789-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13789-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13789-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13789-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13789-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13789-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13789-1-AP (RAP2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 13789-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 13789-1-AP (RAP2A antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules K-562, cellules PC-3, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

13789-1-AP cible RAP2A dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAP2A Protéine recombinante Ag4808
• Nom complet: RAP2A, member of RAS oncogene family
• Masse moléculaire calculée: 183 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC041333
• Symbole du gène: RAP2A
• Identification du gène (NCBI): 5911
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Sci Rep; miR-9 and miR-124 synergistically affect regulation of dendritic branching via the AKT/GSK3β pathway by targeting Rap2a.; Authors - Qian Xue
human	IHC, WB	Clin Exp Med; RAP2A promotes apoptosis resistance of hepatocellular carcinoma cells via the mTOR pathway.; Authors - Jing-Ru Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (05-29-2019)

RAP2A should be 21kD, no prominent bands appeared at that molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

RAP2A Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:13789-1-AP)