Anticorps Recombinant de lapin anti-RAPGEF2

RAPGEF2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241637G5

N° de cat : 84331-4-PBS

Synonymes

PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313, CNrasGEF

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Galerie de données de validation

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  WB analysis using 84331-4-RR (same clone as 84331-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84331-4-RR (RAPGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84331-4-RR (same clone as 84331-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84331-4-RR (RAPGEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84331-4-RR (same clone as 84331-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RAPGEF2 antibody (84331-4-RR, Clone: 241637G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84331-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84331-4-RR against Human RAPGEF2 were performed. The affinity constant is 0.19 nM.

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Informations sur le produit

84331-4-PBS cible RAPGEF2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAPGEF2 Protéine recombinante Ag35288
• Nom complet: Rap guanine nucleotide exchange factor (GEF) 2
• Masse moléculaire calculée: 167kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: NM_014247.2
• Symbole du gène: RAPGEF2
• Identification du gène (NCBI): 9693
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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