Anticorps Polyclonal de lapin anti-RARA

RARA Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 10331-1-AP

Synonymes

retinoic acid receptor, alpha, Retinoic acid receptor alpha, RAR-alpha, RAR alpha, RAR

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Galerie de données de validation

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  WB analysis of K-562 using 10331-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10331-1-AP (RARA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10331-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10331-1-AP (RARA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10331-1-AP

  IP result of anti-RARA (IP:10331-1-AP, 4ug; Detection:10331-1-AP 1:2000) with HeLa cells lysate 1760 ug.

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10331-1-AP cible RARA dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RARA Protéine recombinante Ag0420
• Nom complet: retinoic acid receptor, alpha
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC008727
• Symbole du gène: RARA
• Identification du gène (NCBI): 5914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Retinoic acid receptors (RARs) function as nuclear receptors that control gene expression in response to binding of the ligand retinoic acid (RA). Retinoic acid receptor alpha (RARα), also known as NR1B1 (nuclear receptor subfamily 1, group B, member 1), plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Formation of a complex with histone deacetylases might lead to inhibition of RARE DNA element binding and to transcriptional repression (PubMed:28167758). Transcriptional activation and RARE DNA element binding might be supported by the transcription factor KLF2 (PubMed:28167758).

Publications

Species	Application	Title
human	WB	Br J Cancer; All-trans retinoic acid enhances the cytotoxic effect of decitabine on myelodysplastic syndromes and acute myeloid leukaemia by activating the RARα-Nrf2 complex; Authors - Lu Wang
mouse	WB,IHC	Metabolism; Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.; Authors - Shyi-Jang Shin
human	WB	Front Cell Dev Biol; Silybin Prevents Prostate Cancer by Inhibited the ALDH1A1 Expression in the Retinol Metabolism Pathway.; Authors - Ying Jiang
human	IF	Stem Cell Rev Rep; Pulse Activation of Retinoic Acid Receptor Enhances Hematopoietic Stem Cell Homing by Controlling CXCR4 Membrane Presentation; Authors - Nanxi Geng
human	WB	Cell Signal; ATRA-mediated-crosstalk between stellate cells and Kupffer cells inhibits autophagy and promotes NLRP3 activation in acute liver injury.; Authors - Zhen Yu
human	IF	Andrology; A novel SNP in HUWE1 promoter confers increased risk of NOA by affecting the RA/RARα pathway in Chinese individuals; Authors - Xudong Shan

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