Anticorps Polyclonal de lapin anti-RARB

• Phare
• Validé par KD/KO

RARB Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 14013-1-AP

Synonymes

HAP, HBV activated protein, NR1B2, RAR beta, RAR epsilon, RARB, Retinoic acid receptor beta, retinoic acid receptor, beta, RRB2

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Galerie de données de validation

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  WB analysis using 14013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14013-1-AP (RARB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat bladder using 14013-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14013-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse bladder using 14013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 14013-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 14013-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 14013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 14013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 14013-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RARB antibody (14013-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Sp2/0,
• Résultats positifs en IHC: tissu de côlon de souris, tissu cérébral de rat, tissu cérébral de souris, tissu de cancer du pancréas humain, tissu de carcinome à cellules rénales humain, tissu vésical de rat, tissu vésical de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

14013-1-AP cible RARB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RARB Protéine recombinante Ag5119
• Nom complet: retinoic acid receptor, beta
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 37-51 kDa
• Numéro d’acquisition GenBank: BC060794
• Symbole du gène: RARB
• Identification du gène (NCBI): 5915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Retinoic acid receptors (RARs) are important mediators of retinoid signaling in morphogenesis, development, and cell differentiation. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PMID:12554770). They can bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, they act mainly as activators of gene expression due to weak binding to corepressors. RARB is one isotypes of RARs (PMID:12834868).

Publications

Species	Application	Title
human	WB	Antiviral Res; Retinoic acid receptor β, a potential therapeutic target in the inhibition of adenovirus replication.; Authors - Xiaolong Wang ; KD Validated