Anticorps Polyclonal de lapin anti-RARB

• Phare
• Validé par KD/KO

RARB Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14013-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14013-1-AP (same clone as 14013-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14013-1-AP (RARB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of rat bladder using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse bladder using 14013-1-AP (same clone as 14013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 14013-1-AP (RARB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 14013-1-AP (same clone as 14013-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RARB antibody (14013-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14013-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14013-1-PBS cible RARB dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RARB Protéine recombinante Ag5119
• Nom complet: retinoic acid receptor, beta
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 37-51 kDa
• Numéro d’acquisition GenBank: BC060794
• Symbole du gène: RARB
• Identification du gène (NCBI): 5915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Retinoic acid receptors (RARs) are important mediators of retinoid signaling in morphogenesis, development, and cell differentiation. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes (PMID:12554770). They can bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, they act mainly as activators of gene expression due to weak binding to corepressors. RARB is one isotypes of RARs (PMID:12834868).

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