Anticorps Polyclonal de lapin anti-RARG

• Phare
• Validé par KD/KO

RARG Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11424-1-AP

Synonymes

NR1B3, RAR gamma, RARC, RARG, Retinoic acid receptor gamma, retinoic acid receptor, gamma

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Galerie de données de validation

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  WB analysis of HeLa using 11424-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11424-1-AP (RARG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11424-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11424-1-AP (RARG Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 11424-1-AP

  IP Result of anti-RARG (IP:11424-1-AP, 4ug; Detection:11424-1-AP 1:300) with MCF-7 cells lysate 1120ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

11424-1-AP cible RARG dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RARG Protéine recombinante Ag1991
• Nom complet: retinoic acid receptor, gamma
• Masse moléculaire calculée: 454 aa, 50 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC019098
• Symbole du gène: RARG
• Identification du gène (NCBI): 5916
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Retinoic acid receptors (RARs) are nuclear hormone receptors that act as ligand-dependent transcriptional regulators. In their liganded state, RARs activate transcription, whereas in their nonliganded form, they repress transcription of their target genes. RARs have numerous target genes, which have retinoic response elements in their promoter regions [PMID:17574023]. RARG is a member of the retinoid acid receptor (RAR) family, along with RARA, which is known to be involved PML/RARA fusion of acute promyelocytic leukemia, and RARB. and rearrangement that conferred to leukemic cells acute promyelocytic leukemia-like morphologic and immunophenotypic features [PMID:20935257].

Publications

Species	Application	Title
mouse	ChIP	J Clin Invest; The orphan nuclear receptor small heterodimer partner mediates male infertility induced by diethylstilbestrol in mice.; Authors - Volle David H DH
human	WB,IHC	Front Oncol; High expression of RARG accelerates ovarian cancer progression by regulating cell proliferation; Authors - Lin Xiu ; KD Validated
human	IF	Endocrinology; De novo-synthesized retinoic acid in ovarian antral follicles enhances FSH-mediated ovarian follicular cell differentiation and female fertility.; Authors - Tomoko Kawai
mouse	ChIP	Adv Sci (Weinh); Synthetic Retinoid Kills Drug-Resistant Cancer Stem Cells via Inducing RARγ-Translocation-Mediated Tension Reduction and Chromatin Decondensation; Authors - Yao Zhang