Anticorps Polyclonal de lapin anti-Chemerin

• Phare
• Validé par KD/KO

Chemerin Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 10216-1-AP

Synonymes

Chemerin, HP10433, RAR responsive protein TIG2, RARRES2, TIG2

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Galerie de données de validation

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  WB analysis of human plasma using 10216-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 10216-1-AP (Chemerin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 10216-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10216-1-AP (Chemerin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10216-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10216-1-AP (Chemerin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10216-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10216-1-AP (Chemerin antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10216-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10216-1-AP (Chemerin antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain
• Résultats positifs en IHC: tissu placentaire humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Informations sur le produit

10216-1-AP cible Chemerin dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Chemerin Protéine recombinante Ag0261
• Nom complet: retinoic acid receptor responder (tazarotene induced) 2
• Masse moléculaire calculée: 163 aa, 19 kDa
• Poids moléculaire observé: 14-19 kDa
• Numéro d’acquisition GenBank: BC000069
• Symbole du gène: RARRES2
• Identification du gène (NCBI): 5919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chemerin, also named as TIG2 and RARRES2, is adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). It positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. RARRES2 have both pro- and anti-inflammatory properties depending on the modality of enzymatic cleavage by different classes of proteases. The MW of Chemerin is 14-19 kDa.

Publications

Species	Application	Title
mouse,human	IHC,WB	Mil Med Res; RARRES2 regulates lipid metabolic reprogramming to mediate the development of brain metastasis in triple negative breast cancer; Authors - Yi-Qun Li
human	WB	Cancer Cell Int; Targeting copper death genotyping associated gene RARRES2 suppresses glioblastoma progression and macrophages infiltration; Authors - Tao Yan
human,mouse	WB,IHC	Front Pharmacol; Targeting the chemerin/CMKLR1 axis by small molecule antagonist α-NETA mitigates endometriosis progression; Authors - Ming Yu ; KO Validated
human	IHC	Front Oncol; Neutrophils Promote Tumor Progression in Oral Squamous Cell Carcinoma by Regulating EMT and JAK2/STAT3 Signaling Through Chemerin.; Authors - Xiaoyuan Hu
human	WB	J Zhejiang Univ Sci B; Silencing of DsbA-L gene impairs the PPARγ agonist function of improving insulin resistance in a high-glucose cell model.; Authors - Xuan Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Owen (Verified Customer) (10-07-2019)

The antibody manages to detect the correct band of the protein and is specific. Further optimization for IHC would be great.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: 786-O cell