Anticorps Polyclonal de lapin anti-RARS

• Phare
• Validé par KD/KO

RARS Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 27344-1-AP

Synonymes

Arginine tRNA ligase, arginyl tRNA synthetase, ArgRS, DALRD1, RARS

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Galerie de données de validation

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  WB analysis of HeLa using 27344-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27344-1-AP

  WB result of RARS antibody (27344-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RARS transfected HeLa cells.

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  WB analysis of A431 using 27344-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 27344-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 27344-1-AP (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 27344-1-AP

  IP result of anti-RARS (IP:27344-1-AP, 4ug; Detection:27344-1-AP 1:500) with HeLa cells lysate 3200 ug.

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  IHC staining of human heart using 27344-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 27344-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 27344-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27344-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27344-1-AP (RARS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules U-251
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below

Informations sur le produit

27344-1-AP cible RARS dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RARS Protéine recombinante Ag26390
• Nom complet: arginyl-tRNA synthetase
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC000528
• Symbole du gène: RARS
• Identification du gène (NCBI): 5917
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.

Publications

Species	Application	Title
human	WB	Mol Cell; Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants; Authors - Chao Yang ; KD Validated
human	WB	Mol Cell; A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation; Authors - Raja H Ali
human	WB	bioRxiv; Arginine limitation causes a directed DNA sequence evolution response in colorectal cancer cells; Authors - Dennis J Hsu ; KD Validated
human	WB	Sci Adv; Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells; Authors - Dennis J Hsu ; KD Validated
rat	WB	Mol Neurobiol; Verification of Pain-Related Neuromodulation Mechanisms of Calcitonin in Knee Osteoarthritis; Authors - Qing Lin

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