Anticorps Monoclonal anti-RARS

• Phare
• Validé par KD/KO

RARS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C8G4

N° de cat : 66815-1-PBS

Synonymes

DALRD1, ArgRS, Arginyl-tRNA synthetase, arginyl tRNA synthetase, Arginine--tRNA ligase, cytoplasmic

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Galerie de données de validation

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  WB analysis using 66815-1-Ig (same clone as 66815-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

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  WB analysis using 66815-1-Ig (same clone as 66815-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66815-1-Ig (RARS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66815-1-Ig (same clone as 66815-1-PBS)

  WB result of RARS antibody (66815-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RARS transfected HeLa cells. This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66815-1-Ig (same clone as 66815-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66815-1-Ig (RARS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66815-1-Ig (same clone as 66815-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RARS antibody (66815-1-Ig, Clone: 1C8G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66815-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66815-1-PBS cible RARS dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RARS Protéine recombinante Ag26399
• Nom complet: arginyl-tRNA synthetase
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC000528
• Symbole du gène: RARS
• Identification du gène (NCBI): 5917
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RARS, also named ArgRS (arginyl-tRNA synthetase), belongs to the class-I aminoacyl-tRNA synthetase family which catalyze the aminoacylation of tRNA by their cognate amino acid. In higher eukaryotes, one of the two arginyl-tRNA synthetases (ArgRSs) has evolved to have an extended N-terminal domain that plays a crucial role in protein synthesis and cell growth and in integration into the multisynthetase complex (MSC) (PMID:25288775). RARS has two isoforms with the molecular weight of 75 and 67 kDa.

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