Anticorps Polyclonal de lapin anti-RASGAP

RASGAP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 12935-1-AP

Synonymes

RASA1, Ras p21 protein activator, Ras GTPase-activating protein 1, p120RASGAP, p120GAP

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Galerie de données de validation

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  WB analysis using 12935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12935-1-AP (RASGAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12935-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12935-1-AP (RASGAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12935-1-AP

  IP result of anti-RASGAP (IP:12935-1-AP, 4ug; Detection:12935-1-AP 1:4000) with mouse testis tissue lysate 1120 ug.

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  IHC staining of human testis using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 12935-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12935-1-AP (RASGAP antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12935-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RASGAP antibody (12935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

12935-1-AP cible RASGAP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RASGAP Protéine recombinante Ag3906
• Nom complet: RAS p21 protein activator (GTPase activating protein) 1
• Masse moléculaire calculée: 1047 aa, 116 kDa
• Poids moléculaire observé: 120 kDa, 95 kDa
• Numéro d’acquisition GenBank: BC033015
• Symbole du gène: RASA1
• Identification du gène (NCBI): 5921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	Aging (Albany NY); LncRNA-HAGLR motivates triple negative breast cancer progression by regulation of WNT2 via sponging miR-335-3p.; Authors - Liting Jin
	WB	Am J Transl Res; GRK2 deletion improves the function of skin flap following ischemia-reperfusion injury by regulating Drp1.; Authors - Yapeng Wang
rat	WB	Biochem Biophys Res Commun; miR-335 is involved in the rat epididymal development by targeting the mRNA of RASA1.; Authors - Wang Junfeng J
human	WB	Int J Clin Exp Pathol; Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.; Authors - Xiaojuan Ye
		Funct Integr Genomics; Amlodipine inhibits Synaptotagmin-4's oncogenic activity on gastric cancer proliferation by targeting calcium signaling; Authors - Wen Huang
human	WB	Cancer Res; Blocking Nitrosylation Induces Immunogenic Cell Death by Sensitizing NRAS-Mutant Melanoma to MEK Inhibitors; Authors - Jyoti Srivastava

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