Anticorps Polyclonal de lapin anti-RASA2

RASA2 Polyclonal Antibody for IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31586-1-PBS

Synonymes

GAP1M, RAS p21 protein activator 2, RASGAP

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Galerie de données de validation

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  IP experiment of Jurkat using 31586-1-AP (same clone as 31586-1-PBS)

  IP result of anti-RASA2 (IP:31586-1-AP, 4ug; Detection:31586-1-AP 1:1000) with Jurkat cells lysate 1920 ug. This data was developed using the same antibody clone with 31586-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 31586-1-AP (same clone as 31586-1-PBS)

  IP result of anti-RASA2 (IP:31586-1-AP, 4ug; Detection:31586-1-AP 1:1000) with A431 cells lysate 1120 ug. This data was developed using the same antibody clone with 31586-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 31586-1-AP (same clone as 31586-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASA2 antibody (31586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31586-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31586-1-PBS cible RASA2 dans les applications de IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RASA2 Protéine recombinante Ag35885
• Nom complet: RAS p21 protein activator 2
• Masse moléculaire calculée: 96kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: NM_001303245
• Symbole du gène: RASA2
• Identification du gène (NCBI): 5922
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ras GTPase-activating protein 2 (RASA2) is also named GAP1M and RASGAP. RASA2 was a critical RAS-specific GAP, inhibition of which permitted increased proliferation following TCR stimulation under conditions of immunosuppression (PMID: 37858490). RASA2 was identified as an inhibitor of RAS signaling in multiple human tumors, including melanoma, where inactivating RASA2 mutations have been found (PMID: 26502337). RASA2 is also one of the genes mutated in Noonan syndrome, a Rasopathy associated with developmental defects and elevated RAS activity (PMID: 25049390). RASA2 is expressed in both CD4+ and CD8+ T cells (PMID: 36002574).

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