Anticorps Monoclonal anti-RASEF

RASEF Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C2B2

N° de cat : 67159-1-PBS

Synonymes

FLJ31614, RAB45, Ras related protein Rab 45, RASEF

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Galerie de données de validation

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  WB analysis of LNCaP using 67159-1-Ig (same clone as 67159-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67159-1-Ig (RASEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67159-1-Ig (same clone as 67159-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASEF antibody (67159-1-Ig, Clone: 1C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67159-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67159-1-PBS cible RASEF dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RASEF Protéine recombinante Ag2149
• Nom complet: RAS and EF-hand domain containing
• Masse moléculaire calculée: 740 aa, 83 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC023566
• Symbole du gène: RASEF
• Identification du gène (NCBI): 158158
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.

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