Anticorps Polyclonal de lapin anti-RASGRP2

RASGRP2 Polyclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 30189-1-AP

Synonymes

GRP2, F25B3.3 kinase-like protein, Cdc25-like protein, CDC25L, CalDAG-GEFI

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Galerie de données de validation

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  WB analysis using 30189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30189-1-AP (RASGRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 30189-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RASGRP2 antibody (30189-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of Jurkat using 30189-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human RASGRP2 (30189-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MOLT-4, cellules Raji
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

30189-1-AP cible RASGRP2 dans les applications de WB, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RASGRP2 Protéine recombinante Ag32762
• Nom complet: RAS guanyl releasing protein 2 (calcium and DAG-regulated)
• Masse moléculaire calculée: 69kd
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: NM_153819
• Symbole du gène: RASGRP2
• Identification du gène (NCBI): 10235
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RASGRP2, also named as CDC25L and MCG7, belongs to the RASGRP family. It functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. RASGRP2 may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. RASGRO2 functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. It may function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. The antibody recognizes the C-term of RASGRP2.

Publications

Species	Application	Title
human	WB,CoIP	Biochim Biophys Acta Mol Cell Res; NEDD4L-mediated RASGRP2 suppresses high-glucose and oxLDL-induced vascular endothelial cell dysfunctions by activating Rap1 and R-Ras; Authors - Guozhu Chen

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