Anticorps Monoclonal anti-RASGRP3

RASGRP3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A10G4

N° de cat : 66650-1-PBS

Synonymes

GRP3, KIAA0846, Ras guanyl releasing protein 3, RAS-GRP, RASGRP3

---

Galerie de données de validation

• 

  WB analysis using 66650-1-Ig (same clone as 66650-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66650-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66650-1-Ig (same clone as 66650-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66650-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66650-1-Ig (same clone as 66650-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66650-1-Ig (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66650-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 66650-1-Ig (same clone as 66650-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RASGRP3 antibody (66650-1-Ig, Clone: 1A10G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66650-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66650-1-PBS cible RASGRP3 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RASGRP3 Protéine recombinante Ag3810
• Nom complet: RAS guanyl releasing protein 3 (calcium and DAG-regulated)
• Masse moléculaire calculée: 690 aa, 78 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC027849
• Symbole du gène: RASGRP3
• Identification du gène (NCBI): 25780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt’s lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).

{{ptg:RelatedPrimaryAntibodies}}