Anticorps Recombinant de lapin anti-RB1CC1

RB1CC1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242204D6

N° de cat : 84757-3-PBS

Synonymes

242204D6, CC1, FAK family kinase-interacting protein of 200 kDa, KIAA0203, RB1CC 1

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Galerie de données de validation

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  WB analysis using 84757-3-RR (same clone as 84757-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84757-3-RR (RB1CC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84757-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84757-3-RR (same clone as 84757-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84757-3-RR (RB1CC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84757-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84757-3-RR (same clone as 84757-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84757-3-RR (RB1CC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84757-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84757-3-RR (same clone as 84757-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RB1CC1 antibody (84757-3-RR, Clone: 242204D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84757-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84757-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84757-3-RR against Human RB1CC1 were performed. The affinity constant is 3.20 nM.

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Informations sur le produit

84757-3-PBS cible RB1CC1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RB1CC1 Protéine recombinante Ag10508
• Nom complet: RB1-inducible coiled-coil 1
• Masse moléculaire calculée: 1594 aa, 183 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC017556
• Symbole du gène: RB1CC1
• Identification du gène (NCBI): 9821
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene.

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