Anticorps Monoclonal anti-RBAP48

RBAP48 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

5C4D6

N° de cat : CL594-66060

Synonymes

CAF 1 subunit C, CAF I 48 kDa subunit, CAF I p48, Histone binding protein RBBP4, NURF55, RBAP48, RBBP 4, RBBP4

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Galerie de données de validation

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  IF Staining of A549 using CL594-66060

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594-conjugated RBAP48 antibody (CL594-66060, Clone: 5C4D6 ) at dilution of 1:200, CL488-Phalloidin (green).

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  FC experiment of HepG2 using CL594-66060

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human RBAP48 (CL594-66060, Clone:5C4D6) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of A549 using CL594-66060

  1X10^6 A549 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human RBAP48 (CL594-66060, Clone:5C4D6) (red), or 0.2 ug Control Antibody (CL594-66360-3, Clone: ) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF: cellules A549,
• Résultats positifs en cytométrie: cellules HepG2, cellules A549

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66060 cible RBAP48 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RBAP48 Protéine recombinante Ag6196
• Nom complet: retinoblastoma binding protein 4
• Masse moléculaire calculée: 48 kDa
• Numéro d’acquisition GenBank: BC053904
• Symbole du gène: RBBP4
• Identification du gène (NCBI): 5928
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011