Anticorps Polyclonal de lapin anti-RBAP48

RBAP48 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 14557-1-AP

Synonymes

RBBP4, NURF55, Nucleosome-remodeling factor subunit RBAP48, Histone-binding protein RBBP4, CAF-I p48

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Galerie de données de validation

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  WB analysis of HeLa using 14557-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14557-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14557-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14557-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14557-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14557-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBAP48 antibody (14557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, tissu de thymus de souris
• Résultats positifs en IHC: tissu ovarien humain, tissu cérébral humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14557-1-AP cible RBAP48 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBAP48 Protéine recombinante Ag6110
• Nom complet: retinoblastoma binding protein 4
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 40 kDa, 48-53 kDa
• Numéro d’acquisition GenBank: BC053904
• Symbole du gène: RBBP4
• Identification du gène (NCBI): 5928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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