Anticorps Monoclonal anti-RBAP48

• Phare
• Validé par KD/KO

RBAP48 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

5C4D6

N° de cat : 66060-1-Ig

Synonymes

Histone binding protein RBBP4, RBAP48

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Galerie de données de validation

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  WB analysis using 66060-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66060-1-Ig (RBAP48 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66060-1-Ig

  WB result of RBAP48 antibody (66060-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBAP48 transfected HeLa cells.

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  WB analysis of HeLa using 66060-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66060-1-Ig (RBAP48 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 66060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66060-1-Ig(RBAP48 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 66060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66060-1-Ig(RBAP48 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 66060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66060-1-Ig(RBAP48 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 66060-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66060-1-Ig(RBAP48 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66060-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66060-1-Ig (RBAP48 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66060-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human RBAP48 (66060-1-Ig, Clone:5C4D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules L02, cellules NIH/3T3, cellules RAW 264.7, cellules ROS1728
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66060-1-Ig cible RBAP48 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RBAP48 Protéine recombinante Ag6196
• Nom complet: retinoblastoma binding protein 4
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC053904
• Symbole du gène: RBBP4
• Identification du gène (NCBI): 5928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone-binding protein RBBP4 (also known as RbAp48, or NURF55) is a protein that in humans is encoded by the RBBP4 gene. This gene encodes a ubiquitously expressed nuclear protein that belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2/NuRD complex complex that has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of corepressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. A decrease of RbAp48 in the dentate gyrus (DG) of the hippocampus in the brain is suspected to be a main cause of memory loss in normal aging (PMID: 23986399).

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011